Partial purification and some properties of delta1-pyrroline-5-carboxylate reductase from Escherichia coli
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چکیده
منابع مشابه
Pyrroline-5-carboxylate reductase of Neurospora crassa; partial purification and some properties.
Previous studies (1, 2) with mutant and wild type strains of Neurospora crassa have pointed to glutamic y-semialdehyde and its spontaneously cyclized form, Al-pyrroline-5-carboxylate, as intermediates in the biosynthesis of proline. These studies, based largely on experiments in vivo, have recently been substantiated through work in vitro with extracted enzymes (3, 4). ‘The terminal step in pro...
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Pyrroline-5-carboxylate reductase (P5CR) catalyzes the reduction of Delta1-pyrroline-5-carboxylate (P5C) to proline with concomitant oxidation of NAD(P)H to NAD(P)(+). The enzymatic cycle between P5C and proline is very important in many physiological and pathological processes. Human P5CR was over-expressed in Escherichia coli and purified to homogeneity by chromatography. Enzymatic assays of ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1977
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.129.1.108-114.1977